n terminal sv40 nls sequence present Search Results


n terminal sv40 nls sequence present  (Addgene inc)
95
Addgene inc n terminal sv40 nls sequence present
N Terminal Sv40 Nls Sequence Present, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sv40 tantigen  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology sv40 tantigen
(A) Detection of H-Ras, c-myc, and Bcl-2 by Western blot analysis in primary PATAS cells, BARF1-transfected PATAS (P-BA), BARF1 and Ras-transfected PATAS (P-BA-R), hTERT-transfected PATAS (P-TE), and hTERT and Ras-transfected PATAS cells (P-TE-R). (B) Foci formation was detected by light microscopy. Whereas BARF1-transfected PATAS cells showed an organized growth (photographed at x50 [a] and x100 [c]), PATAS expressing both BARF1 and Ras genes formed a higher number of large foci (photographed at x50 [b] and x100 [d]). Cells were cultured in DMEM supplemented with 10% serum. (C) Schematic diagram of the <t>SV40</t> ER and the alternatively spliced SV40 viral transcripts coding for large T, small T, and 17K T proteins. Numbering refers to the SV40 genomic nucleotide numbers. Origin of replication was indicated as Ori. (D) PCR for the detection of the alternatively spliced messenger RNA encoding the SV40 ST. Positions of PCR primers are indicated in panel A. m, marker. a, PCR with NP69 cell DNA to amplify SV40 ER including LT exons and intron. b, RT-PCR with NP69 cell RNA to amplify SV40 LT transcript. c, RT-PCR with NP69 cell RNA to amplify SV40 ST transcript. d, RT-PCR with 293T cell RNA to amplify SV40 LT transcript. e, PCR with 293 cell DNA as negative control.
Sv40 Tantigen, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c terminal sv40 nls sequences  (Addgene inc)
85
Addgene inc c terminal sv40 nls sequences
(A) Detection of H-Ras, c-myc, and Bcl-2 by Western blot analysis in primary PATAS cells, BARF1-transfected PATAS (P-BA), BARF1 and Ras-transfected PATAS (P-BA-R), hTERT-transfected PATAS (P-TE), and hTERT and Ras-transfected PATAS cells (P-TE-R). (B) Foci formation was detected by light microscopy. Whereas BARF1-transfected PATAS cells showed an organized growth (photographed at x50 [a] and x100 [c]), PATAS expressing both BARF1 and Ras genes formed a higher number of large foci (photographed at x50 [b] and x100 [d]). Cells were cultured in DMEM supplemented with 10% serum. (C) Schematic diagram of the <t>SV40</t> ER and the alternatively spliced SV40 viral transcripts coding for large T, small T, and 17K T proteins. Numbering refers to the SV40 genomic nucleotide numbers. Origin of replication was indicated as Ori. (D) PCR for the detection of the alternatively spliced messenger RNA encoding the SV40 ST. Positions of PCR primers are indicated in panel A. m, marker. a, PCR with NP69 cell DNA to amplify SV40 ER including LT exons and intron. b, RT-PCR with NP69 cell RNA to amplify SV40 LT transcript. c, RT-PCR with NP69 cell RNA to amplify SV40 ST transcript. d, RT-PCR with 293T cell RNA to amplify SV40 LT transcript. e, PCR with 293 cell DNA as negative control.
C Terminal Sv40 Nls Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cxcr2 antibody  (Boster Bio)
93
Boster Bio cxcr2 antibody
(A) Detection of H-Ras, c-myc, and Bcl-2 by Western blot analysis in primary PATAS cells, BARF1-transfected PATAS (P-BA), BARF1 and Ras-transfected PATAS (P-BA-R), hTERT-transfected PATAS (P-TE), and hTERT and Ras-transfected PATAS cells (P-TE-R). (B) Foci formation was detected by light microscopy. Whereas BARF1-transfected PATAS cells showed an organized growth (photographed at x50 [a] and x100 [c]), PATAS expressing both BARF1 and Ras genes formed a higher number of large foci (photographed at x50 [b] and x100 [d]). Cells were cultured in DMEM supplemented with 10% serum. (C) Schematic diagram of the <t>SV40</t> ER and the alternatively spliced SV40 viral transcripts coding for large T, small T, and 17K T proteins. Numbering refers to the SV40 genomic nucleotide numbers. Origin of replication was indicated as Ori. (D) PCR for the detection of the alternatively spliced messenger RNA encoding the SV40 ST. Positions of PCR primers are indicated in panel A. m, marker. a, PCR with NP69 cell DNA to amplify SV40 ER including LT exons and intron. b, RT-PCR with NP69 cell RNA to amplify SV40 LT transcript. c, RT-PCR with NP69 cell RNA to amplify SV40 ST transcript. d, RT-PCR with 293T cell RNA to amplify SV40 LT transcript. e, PCR with 293 cell DNA as negative control.
Cxcr2 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+sv40+nls+sequence+present/Anti-CXCR2+Antibody/ppr0258207-81-30-35
Average 93 stars, based on 1 article reviews
cxcr2 antibody - by Bioz Stars, 2026-09
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paper n a peft  (Addgene inc)
95
Addgene inc paper n a peft
(A) Detection of H-Ras, c-myc, and Bcl-2 by Western blot analysis in primary PATAS cells, BARF1-transfected PATAS (P-BA), BARF1 and Ras-transfected PATAS (P-BA-R), hTERT-transfected PATAS (P-TE), and hTERT and Ras-transfected PATAS cells (P-TE-R). (B) Foci formation was detected by light microscopy. Whereas BARF1-transfected PATAS cells showed an organized growth (photographed at x50 [a] and x100 [c]), PATAS expressing both BARF1 and Ras genes formed a higher number of large foci (photographed at x50 [b] and x100 [d]). Cells were cultured in DMEM supplemented with 10% serum. (C) Schematic diagram of the <t>SV40</t> ER and the alternatively spliced SV40 viral transcripts coding for large T, small T, and 17K T proteins. Numbering refers to the SV40 genomic nucleotide numbers. Origin of replication was indicated as Ori. (D) PCR for the detection of the alternatively spliced messenger RNA encoding the SV40 ST. Positions of PCR primers are indicated in panel A. m, marker. a, PCR with NP69 cell DNA to amplify SV40 ER including LT exons and intron. b, RT-PCR with NP69 cell RNA to amplify SV40 LT transcript. c, RT-PCR with NP69 cell RNA to amplify SV40 ST transcript. d, RT-PCR with 293T cell RNA to amplify SV40 LT transcript. e, PCR with 293 cell DNA as negative control.
Paper N A Peft, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sv40 large t antigen nuclear localization signal pkkkrkv  (Addgene inc)
94
Addgene inc sv40 large t antigen nuclear localization signal pkkkrkv
(A) Detection of H-Ras, c-myc, and Bcl-2 by Western blot analysis in primary PATAS cells, BARF1-transfected PATAS (P-BA), BARF1 and Ras-transfected PATAS (P-BA-R), hTERT-transfected PATAS (P-TE), and hTERT and Ras-transfected PATAS cells (P-TE-R). (B) Foci formation was detected by light microscopy. Whereas BARF1-transfected PATAS cells showed an organized growth (photographed at x50 [a] and x100 [c]), PATAS expressing both BARF1 and Ras genes formed a higher number of large foci (photographed at x50 [b] and x100 [d]). Cells were cultured in DMEM supplemented with 10% serum. (C) Schematic diagram of the <t>SV40</t> ER and the alternatively spliced SV40 viral transcripts coding for large T, small T, and 17K T proteins. Numbering refers to the SV40 genomic nucleotide numbers. Origin of replication was indicated as Ori. (D) PCR for the detection of the alternatively spliced messenger RNA encoding the SV40 ST. Positions of PCR primers are indicated in panel A. m, marker. a, PCR with NP69 cell DNA to amplify SV40 ER including LT exons and intron. b, RT-PCR with NP69 cell RNA to amplify SV40 LT transcript. c, RT-PCR with NP69 cell RNA to amplify SV40 ST transcript. d, RT-PCR with 293T cell RNA to amplify SV40 LT transcript. e, PCR with 293 cell DNA as negative control.
Sv40 Large T Antigen Nuclear Localization Signal Pkkkrkv, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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sv40 nuclear localization sequences nls  (Addgene inc)
93
Addgene inc sv40 nuclear localization sequences nls
(A) Detection of H-Ras, c-myc, and Bcl-2 by Western blot analysis in primary PATAS cells, BARF1-transfected PATAS (P-BA), BARF1 and Ras-transfected PATAS (P-BA-R), hTERT-transfected PATAS (P-TE), and hTERT and Ras-transfected PATAS cells (P-TE-R). (B) Foci formation was detected by light microscopy. Whereas BARF1-transfected PATAS cells showed an organized growth (photographed at x50 [a] and x100 [c]), PATAS expressing both BARF1 and Ras genes formed a higher number of large foci (photographed at x50 [b] and x100 [d]). Cells were cultured in DMEM supplemented with 10% serum. (C) Schematic diagram of the <t>SV40</t> ER and the alternatively spliced SV40 viral transcripts coding for large T, small T, and 17K T proteins. Numbering refers to the SV40 genomic nucleotide numbers. Origin of replication was indicated as Ori. (D) PCR for the detection of the alternatively spliced messenger RNA encoding the SV40 ST. Positions of PCR primers are indicated in panel A. m, marker. a, PCR with NP69 cell DNA to amplify SV40 ER including LT exons and intron. b, RT-PCR with NP69 cell RNA to amplify SV40 LT transcript. c, RT-PCR with NP69 cell RNA to amplify SV40 ST transcript. d, RT-PCR with 293T cell RNA to amplify SV40 LT transcript. e, PCR with 293 cell DNA as negative control.
Sv40 Nuclear Localization Sequences Nls, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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α sv40 t ag n terminus  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology α sv40 t ag n terminus
(A) Detection of H-Ras, c-myc, and Bcl-2 by Western blot analysis in primary PATAS cells, BARF1-transfected PATAS (P-BA), BARF1 and Ras-transfected PATAS (P-BA-R), hTERT-transfected PATAS (P-TE), and hTERT and Ras-transfected PATAS cells (P-TE-R). (B) Foci formation was detected by light microscopy. Whereas BARF1-transfected PATAS cells showed an organized growth (photographed at x50 [a] and x100 [c]), PATAS expressing both BARF1 and Ras genes formed a higher number of large foci (photographed at x50 [b] and x100 [d]). Cells were cultured in DMEM supplemented with 10% serum. (C) Schematic diagram of the <t>SV40</t> ER and the alternatively spliced SV40 viral transcripts coding for large T, small T, and 17K T proteins. Numbering refers to the SV40 genomic nucleotide numbers. Origin of replication was indicated as Ori. (D) PCR for the detection of the alternatively spliced messenger RNA encoding the SV40 ST. Positions of PCR primers are indicated in panel A. m, marker. a, PCR with NP69 cell DNA to amplify SV40 ER including LT exons and intron. b, RT-PCR with NP69 cell RNA to amplify SV40 LT transcript. c, RT-PCR with NP69 cell RNA to amplify SV40 ST transcript. d, RT-PCR with 293T cell RNA to amplify SV40 LT transcript. e, PCR with 293 cell DNA as negative control.
α Sv40 T Ag N Terminus, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n+terminal+sv40+nls+sequence+present/SV40/pmc03816221-17-0-9
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renilla luciferase reporter driven by the sv-40 promoter  (Promega)
90
Promega renilla luciferase reporter driven by the sv-40 promoter
(A) Detection of H-Ras, c-myc, and Bcl-2 by Western blot analysis in primary PATAS cells, BARF1-transfected PATAS (P-BA), BARF1 and Ras-transfected PATAS (P-BA-R), hTERT-transfected PATAS (P-TE), and hTERT and Ras-transfected PATAS cells (P-TE-R). (B) Foci formation was detected by light microscopy. Whereas BARF1-transfected PATAS cells showed an organized growth (photographed at x50 [a] and x100 [c]), PATAS expressing both BARF1 and Ras genes formed a higher number of large foci (photographed at x50 [b] and x100 [d]). Cells were cultured in DMEM supplemented with 10% serum. (C) Schematic diagram of the <t>SV40</t> ER and the alternatively spliced SV40 viral transcripts coding for large T, small T, and 17K T proteins. Numbering refers to the SV40 genomic nucleotide numbers. Origin of replication was indicated as Ori. (D) PCR for the detection of the alternatively spliced messenger RNA encoding the SV40 ST. Positions of PCR primers are indicated in panel A. m, marker. a, PCR with NP69 cell DNA to amplify SV40 ER including LT exons and intron. b, RT-PCR with NP69 cell RNA to amplify SV40 LT transcript. c, RT-PCR with NP69 cell RNA to amplify SV40 ST transcript. d, RT-PCR with 293T cell RNA to amplify SV40 LT transcript. e, PCR with 293 cell DNA as negative control.
Renilla Luciferase Reporter Driven By The Sv 40 Promoter, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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simian virus 40  (Biochemie GmbH)
90
Biochemie GmbH simian virus 40
(A) Detection of H-Ras, c-myc, and Bcl-2 by Western blot analysis in primary PATAS cells, BARF1-transfected PATAS (P-BA), BARF1 and Ras-transfected PATAS (P-BA-R), hTERT-transfected PATAS (P-TE), and hTERT and Ras-transfected PATAS cells (P-TE-R). (B) Foci formation was detected by light microscopy. Whereas BARF1-transfected PATAS cells showed an organized growth (photographed at x50 [a] and x100 [c]), PATAS expressing both BARF1 and Ras genes formed a higher number of large foci (photographed at x50 [b] and x100 [d]). Cells were cultured in DMEM supplemented with 10% serum. (C) Schematic diagram of the <t>SV40</t> ER and the alternatively spliced SV40 viral transcripts coding for large T, small T, and 17K T proteins. Numbering refers to the SV40 genomic nucleotide numbers. Origin of replication was indicated as Ori. (D) PCR for the detection of the alternatively spliced messenger RNA encoding the SV40 ST. Positions of PCR primers are indicated in panel A. m, marker. a, PCR with NP69 cell DNA to amplify SV40 ER including LT exons and intron. b, RT-PCR with NP69 cell RNA to amplify SV40 LT transcript. c, RT-PCR with NP69 cell RNA to amplify SV40 ST transcript. d, RT-PCR with 293T cell RNA to amplify SV40 LT transcript. e, PCR with 293 cell DNA as negative control.
Simian Virus 40, supplied by Biochemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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simian virus 40 (sv40) polyadenylation  (GenScript corporation)
90
GenScript corporation simian virus 40 (sv40) polyadenylation
(A) Detection of H-Ras, c-myc, and Bcl-2 by Western blot analysis in primary PATAS cells, BARF1-transfected PATAS (P-BA), BARF1 and Ras-transfected PATAS (P-BA-R), hTERT-transfected PATAS (P-TE), and hTERT and Ras-transfected PATAS cells (P-TE-R). (B) Foci formation was detected by light microscopy. Whereas BARF1-transfected PATAS cells showed an organized growth (photographed at x50 [a] and x100 [c]), PATAS expressing both BARF1 and Ras genes formed a higher number of large foci (photographed at x50 [b] and x100 [d]). Cells were cultured in DMEM supplemented with 10% serum. (C) Schematic diagram of the <t>SV40</t> ER and the alternatively spliced SV40 viral transcripts coding for large T, small T, and 17K T proteins. Numbering refers to the SV40 genomic nucleotide numbers. Origin of replication was indicated as Ori. (D) PCR for the detection of the alternatively spliced messenger RNA encoding the SV40 ST. Positions of PCR primers are indicated in panel A. m, marker. a, PCR with NP69 cell DNA to amplify SV40 ER including LT exons and intron. b, RT-PCR with NP69 cell RNA to amplify SV40 LT transcript. c, RT-PCR with NP69 cell RNA to amplify SV40 ST transcript. d, RT-PCR with 293T cell RNA to amplify SV40 LT transcript. e, PCR with 293 cell DNA as negative control.
Simian Virus 40 (Sv40) Polyadenylation, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Detection of H-Ras, c-myc, and Bcl-2 by Western blot analysis in primary PATAS cells, BARF1-transfected PATAS (P-BA), BARF1 and Ras-transfected PATAS (P-BA-R), hTERT-transfected PATAS (P-TE), and hTERT and Ras-transfected PATAS cells (P-TE-R). (B) Foci formation was detected by light microscopy. Whereas BARF1-transfected PATAS cells showed an organized growth (photographed at x50 [a] and x100 [c]), PATAS expressing both BARF1 and Ras genes formed a higher number of large foci (photographed at x50 [b] and x100 [d]). Cells were cultured in DMEM supplemented with 10% serum. (C) Schematic diagram of the SV40 ER and the alternatively spliced SV40 viral transcripts coding for large T, small T, and 17K T proteins. Numbering refers to the SV40 genomic nucleotide numbers. Origin of replication was indicated as Ori. (D) PCR for the detection of the alternatively spliced messenger RNA encoding the SV40 ST. Positions of PCR primers are indicated in panel A. m, marker. a, PCR with NP69 cell DNA to amplify SV40 ER including LT exons and intron. b, RT-PCR with NP69 cell RNA to amplify SV40 LT transcript. c, RT-PCR with NP69 cell RNA to amplify SV40 ST transcript. d, RT-PCR with 293T cell RNA to amplify SV40 LT transcript. e, PCR with 293 cell DNA as negative control.

Journal: Neoplasia (New York, N.Y.)

Article Title: Synergism of BARF1 with Ras Induces Malignant Transformation in Primary Primate Epithelial Cells and Human Nasopharyngeal Epithelial Cells 1

doi:

Figure Lengend Snippet: (A) Detection of H-Ras, c-myc, and Bcl-2 by Western blot analysis in primary PATAS cells, BARF1-transfected PATAS (P-BA), BARF1 and Ras-transfected PATAS (P-BA-R), hTERT-transfected PATAS (P-TE), and hTERT and Ras-transfected PATAS cells (P-TE-R). (B) Foci formation was detected by light microscopy. Whereas BARF1-transfected PATAS cells showed an organized growth (photographed at x50 [a] and x100 [c]), PATAS expressing both BARF1 and Ras genes formed a higher number of large foci (photographed at x50 [b] and x100 [d]). Cells were cultured in DMEM supplemented with 10% serum. (C) Schematic diagram of the SV40 ER and the alternatively spliced SV40 viral transcripts coding for large T, small T, and 17K T proteins. Numbering refers to the SV40 genomic nucleotide numbers. Origin of replication was indicated as Ori. (D) PCR for the detection of the alternatively spliced messenger RNA encoding the SV40 ST. Positions of PCR primers are indicated in panel A. m, marker. a, PCR with NP69 cell DNA to amplify SV40 ER including LT exons and intron. b, RT-PCR with NP69 cell RNA to amplify SV40 LT transcript. c, RT-PCR with NP69 cell RNA to amplify SV40 ST transcript. d, RT-PCR with 293T cell RNA to amplify SV40 LT transcript. e, PCR with 293 cell DNA as negative control.

Article Snippet: Western Blot Analysis Immunoblot analysis was performed as previously described [ 18 ]; after separation by 8% to 12% polyacrylamide gel electrophoresis, the following antibodies were used to confirm protein expression: primary antibody against Bcl-2 (sc-7382; Santa Cruz, Germany), c- myc (sc-764; Santa Cruz), BARF1 (anti-Pep 2A serum, a polyclonal rabbit antiserum prepared against a synthetic peptide corresponding to a presumed epitope, amino acids (aa) 172 to 180 [NGGVMKEKD], of the BARF1 protein), Ras (sc-29; Santa Cruz), SV40 Tantigen (sc-148; Santa Cruz; N-terminal epitope mapping within residues 1 to 82 of SV40 large Tantigen for detection of 94-kDa SV40 LT antigen and 21-kDa SV40 ST antigen), LMP1 (BD Biosciences Pharmingen, San Diego, CA), h-TERT (SC-7212; Santa-Cruz), and tubulin (sc-5286; Santa Cruz).

Techniques: Western Blot, Transfection, Light Microscopy, Expressing, Cell Culture, Marker, Reverse Transcription Polymerase Chain Reaction, Negative Control

(A) Expression of BARF1 protein and H-Ras in cell lines, tumor biopsies, and tumor cell lines. Cell extracts were prepared from PATAS (primary cells), P-BA (PATAS transfected by BARF1), P-BA-R (P-BA plus Ras), P-BA-R-T (tumor biopsy induced by injection of P-BA-R), and P-BA-R-TL (established cell line from tumor biopsy induced by injection of P-BA-R). For BARF1 detection, A20, BARF1 protein produced by BARF1 recombinant adenovirus system was used as a positive control. For H-Ras detection, we used protein extracted from NPC biopsy expressing a high Ras protein (NPC26) as a positive control. (B) Detection of cytokeratin AE1/AE3 in cell lines. Cells were cultured in microchamber for 72 hours and fixed with acetone for 15 minutes, then anti-AE1/AE3 with a dilution of 50 was treated on each cell. a, Primary PATAS cells. b, P-BA-R cell line. c, P-BA-R tumor cell culture after 4 days (TL1). d, The 30th passage of P-BA-R tumor cell line (TL1). (C) Western blot analysis of associated signaling molecules in BARF1-expressing, LMP1-expressing, and control cell populations. Different cell populations were indicated at the top of the panel. The detected proteins are indicated on the right side. (D) RT-PCR detection of BARF1 and Western blot analysis of Ras and SV40 LT and ST expressions in cloned cell line and tumors. Different tumors formed by NP69-BARF1 + Ras cloned cell line were indicated at the top of the panel. The detected molecules are indicated on the right side.

Journal: Neoplasia (New York, N.Y.)

Article Title: Synergism of BARF1 with Ras Induces Malignant Transformation in Primary Primate Epithelial Cells and Human Nasopharyngeal Epithelial Cells 1

doi:

Figure Lengend Snippet: (A) Expression of BARF1 protein and H-Ras in cell lines, tumor biopsies, and tumor cell lines. Cell extracts were prepared from PATAS (primary cells), P-BA (PATAS transfected by BARF1), P-BA-R (P-BA plus Ras), P-BA-R-T (tumor biopsy induced by injection of P-BA-R), and P-BA-R-TL (established cell line from tumor biopsy induced by injection of P-BA-R). For BARF1 detection, A20, BARF1 protein produced by BARF1 recombinant adenovirus system was used as a positive control. For H-Ras detection, we used protein extracted from NPC biopsy expressing a high Ras protein (NPC26) as a positive control. (B) Detection of cytokeratin AE1/AE3 in cell lines. Cells were cultured in microchamber for 72 hours and fixed with acetone for 15 minutes, then anti-AE1/AE3 with a dilution of 50 was treated on each cell. a, Primary PATAS cells. b, P-BA-R cell line. c, P-BA-R tumor cell culture after 4 days (TL1). d, The 30th passage of P-BA-R tumor cell line (TL1). (C) Western blot analysis of associated signaling molecules in BARF1-expressing, LMP1-expressing, and control cell populations. Different cell populations were indicated at the top of the panel. The detected proteins are indicated on the right side. (D) RT-PCR detection of BARF1 and Western blot analysis of Ras and SV40 LT and ST expressions in cloned cell line and tumors. Different tumors formed by NP69-BARF1 + Ras cloned cell line were indicated at the top of the panel. The detected molecules are indicated on the right side.

Article Snippet: Western Blot Analysis Immunoblot analysis was performed as previously described [ 18 ]; after separation by 8% to 12% polyacrylamide gel electrophoresis, the following antibodies were used to confirm protein expression: primary antibody against Bcl-2 (sc-7382; Santa Cruz, Germany), c- myc (sc-764; Santa Cruz), BARF1 (anti-Pep 2A serum, a polyclonal rabbit antiserum prepared against a synthetic peptide corresponding to a presumed epitope, amino acids (aa) 172 to 180 [NGGVMKEKD], of the BARF1 protein), Ras (sc-29; Santa Cruz), SV40 Tantigen (sc-148; Santa Cruz; N-terminal epitope mapping within residues 1 to 82 of SV40 large Tantigen for detection of 94-kDa SV40 LT antigen and 21-kDa SV40 ST antigen), LMP1 (BD Biosciences Pharmingen, San Diego, CA), h-TERT (SC-7212; Santa-Cruz), and tubulin (sc-5286; Santa Cruz).

Techniques: Expressing, Transfection, Injection, Produced, Recombinant, Positive Control, Cell Culture, Western Blot, Control, Reverse Transcription Polymerase Chain Reaction, Clone Assay

Colony Formation in Soft Agar and Tumor Formation in Nude Mice.

Journal: Neoplasia (New York, N.Y.)

Article Title: Synergism of BARF1 with Ras Induces Malignant Transformation in Primary Primate Epithelial Cells and Human Nasopharyngeal Epithelial Cells 1

doi:

Figure Lengend Snippet: Colony Formation in Soft Agar and Tumor Formation in Nude Mice.

Article Snippet: Western Blot Analysis Immunoblot analysis was performed as previously described [ 18 ]; after separation by 8% to 12% polyacrylamide gel electrophoresis, the following antibodies were used to confirm protein expression: primary antibody against Bcl-2 (sc-7382; Santa Cruz, Germany), c- myc (sc-764; Santa Cruz), BARF1 (anti-Pep 2A serum, a polyclonal rabbit antiserum prepared against a synthetic peptide corresponding to a presumed epitope, amino acids (aa) 172 to 180 [NGGVMKEKD], of the BARF1 protein), Ras (sc-29; Santa Cruz), SV40 Tantigen (sc-148; Santa Cruz; N-terminal epitope mapping within residues 1 to 82 of SV40 large Tantigen for detection of 94-kDa SV40 LT antigen and 21-kDa SV40 ST antigen), LMP1 (BD Biosciences Pharmingen, San Diego, CA), h-TERT (SC-7212; Santa-Cruz), and tubulin (sc-5286; Santa Cruz).

Techniques: Plasmid Preparation